Abstract Background Predicting and proper ranking of canonical splice sites (SSs) is a challenging problem in bioinformatics and machine learning communities. Any progress in SSs recognition will lead to better understanding of splicing mechanism. We introduce several new approaches of combining a priori knowledge for improved SS detection. https://www.roamingfreesims.com/product-category/ansell-lighting-twistlock-50w-fixed-gu10-chrome-die-cast-downlight/